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创赛科技:rHuApo-SAA1细胞因子/重组蛋白领衔销售榜单

      众所周知,每个行业及产品线,都会在每年的时候产生销量优越王,那么细胞因子/重组蛋白里面又有谁成为今年的销量优越王呢?跟随小编一起了解一下。

     在整个销售排行榜单当中,另外们惊讶的发现rHuApo-SAA1这样一个重组蛋白的名字,这个产品对于小编来说,好像只看懂了rHu 代表着重组人,其他好像一概没看明白。但是作为一个勤奋好学的小编,怎么可能就到此为止,翻阅资料检索网络就是*基本的操作,那么一起来看看我的成果吧。

        rHuApo-SAA1,全称Recombinant Human Apolipoprotein-Serum Amyloid A1,翻译出来应该叫做重组人载脂蛋白血清淀粉样蛋白A1,oh my god!中文名字就这么长,我只能说,哪些科学家们,真的厉害!请收下小编的膝盖!(我真的太难了)。

对于这个产品的描述是这样的:

Serum amyloid A proteins(SAA)represents a family of apolipoproteins that circulates in association with high-density lipoproteins(HDL).The level of apo-SAA,normally 1-5μg/ml in plasma,increases 500-1000 fold within 24hoursof an inflammatory stimulus and,under these conditions,is the most abundant HDL apo-lipoprotein.The human SAA gene codes for a 122 amino acid non-glycosylated polypeptide,which contains an 18 amino acid N-terminal sequence.Recombinant human apo-SAA1 is an 11.7 kDa protein containing 104 amino acid residues.

      为了方便大家查看,小编还是翻译了一下,尽可能做到准确,仅供大家参考:

      血清淀粉样蛋白a(saa)是一个载脂蛋白家族,与高密度脂蛋白(hdl)结合循环。在炎症刺激的24小时内,血浆中的apo-saa水平(通常为1-5μg/ml)增加500-1000倍,在这些条件下,是*丰富的hdl-apo-脂蛋白。122个氨基酸非糖基化多肽,含18个氨基酸n末端序列,重组人apo-saa1为11.7kda蛋白,含104个氨基酸残基。

 

Accession

P0DJI8

GeneID

6288

Source

Escherichia coli.

Molecular Weight

Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids.

Quantity

10µg/50µg/1000µg

AA Sequence

RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 9.0, 150 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

 

参考文献看起来:

1.Expression of apolipoprotein serum amyloid A mRNA in human atherosclerotic lesions and cultured vascular cells: implications for serum amyloid A function. 作者:Meek, R. L., Urieli-Shoval, S., Benditt, E. P.

2. Serum amyloid A (SAA): An acute phase protein and apolipoprotein 作者:Ernst Malle,Armin Steinmetz,John G. Raynes

3. [No significant influence of serum amyloid A1 genotypes on serum lipids or HDL clearance] 作者:A Wada,T Yamada,K Itoh...

因为时间关系,就没有给大家整理那么**,大家可以用上面关键词在国内几家数据库中都可以检索到文献进行参考。

 

突然想起来,这种微量的产品保存和使用上是有技巧的:

1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。

2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。

3. 具体的*佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化

 

至此,相信大家对于科学家们,已经是佩服的五体投地了,反正小编是佩服的不要不要的。好啦,这期就先到这里,下期再见啦!