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重组人CD73/5'-Nucleotidase/NT5EProtein(C-6His)

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产品名称: 重组人CD73/5'-Nucleotidase/NT5EProtein(C-6His)
产品型号: PN100005-10ug
产品展商: PERFEMIKER
产品价格: 1200.00 元
会员价格: 0.00 元
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简单介绍

重组人CD73/5'-Nucleotidase/NT5EProtein(C-6His) 货号:PN100005 规格:10ug; 标签:C-6His; 来源:Human; 表达宿主:Human cells


重组人CD73/5'-Nucleotidase/NT5EProtein(C-6His)  的详细介绍

名称:重组人CD73/5'-Nucleotidase/NT5EProtein(C-6His);


产品说明(Description)
Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus. 
Accession #: P21589
Known as: 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE

制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.

质量控制(Quality Control) 
Bioactivity*   Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit
The specific activity is >10,000 pmol/min/μg.
Purity: Greater than 90% as determined by reducing SDS-PAGE.    
Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

复溶(Reconstitution)

保存(Storage)
Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.   

背景(Background)
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

电泳(SDS-PAGE)

FOR RESEARCH USE ONLY