名称:重组人ATG10/Ubiquitin-like-conjugating enzyme ATG10Protein(C-6His, N-T7 tag);
产品说明(Description) Recombinant Human Autophagy Related 10 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Thr190 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus. Accession #: Q9H0Y0 Known as: Ubiquitin-Like-Conjugating Enzyme ATG10; Autophagy-Related Protein 10; APG10-Like; ATG10 制剂(Formulation) Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 8.0. 质量控制(Quality Control) Purity: Greater than 95% as determined by reducing SDS-PAGE. Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. 复溶(Reconstitution) 保存(Storage) Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. 背景(Background) Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7. 电泳(SDS-PAGE) FOR RESEARCH USE ONLY