名称:rStreptavidin
Accession:P22629
Source:Escherichia coli.
Molecular Weight:~52,000 per tetramer.
Quantity:1mg/5mg/1g
AA Sequence:MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
A282 of 0.1% solution:3.1
Purity:> 98 % by SDS-PAGE and HPLC analyses
Specific Activity:> 17 U/mg (one unit binds 1 µg D-biotin at pH 8.9)
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized in 10 mM potassium phosphate buffer pH 6.5.
Endotoxin:Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.
Proteolytic Activity:< 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)
Reconstitution:Dissolve with double distilled water
Clearly soluble in ddH2O (OD405nm < 0.1 at 10 mg/ml water):Storage (lyophilized)
SDS-PAGE: