名称:重组人IL-20
Synonyms:Cytokine Zcyto10
Species:9
Accession:Q9NYY1
GeneID:50604
Source:Escherichia coli.
Molecular Weight:Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity:2µg/10µg/1000µg
AA Sequence:MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
Purity:> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.2, with trehalose.
Endotoxin:Less than 1 EU/µg of rHuIL-20 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference:1. Sabat R. 2010. Cytokine Growth Factor Rev, 21: 315-24.
2. Blumberg H, Conklin D, Xu WF, et al. 2001. Cell, 104: 9-19.
3. Wei CC, Hsu YH, Li HH, et al. 2006. J Biomed Sci, 13: 601-12.
4. Hsu YH, Li HH, Hsieh MY, et al. 2006. Arthritis Rheum, 54: 2722-33.
Background:Human Interleukin-20 (IL-20) is encoded by the IL20 gene located on the chromosome 1 and belongs to the IL-10 family that including IL-10, IL-19, IL-22, IL-24, and IL-26. It is secreted by activated keratinocytes and monocytes, and signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 has functions of regulating proliferation and differentiation of keratinocytes during inflammation, and causing cell expansion of multipotential hematopoietic progenitor cells.