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重组人BMP-7

产品名称: 重组人BMP-7
产品型号: PP100159-2μg
产品展商: PERFEMIKER
产品价格: 600.00 元
会员价格: 0.00 元
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简单介绍

名称:重组人BMP-7 货号:PP100159 规格:2μg


重组人BMP-7  的详细介绍

名称:重组人BMP-7

Synonyms:OP-1

Species:9

Accession:P18075

GeneID:655

Source:Escherichia coli.

Molecular Weight:Approximately 15.7 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids.

Quantity:2µg/10µg/1000µg

AA Sequence:STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity:Data is not available.

Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

Endotoxin:Less than 1 EU/μg of rHuBMP-7 as determined by LAL method.

Applications:1. Molecular standard (Western, ELISA) in studying secreted BMP-7;
2. Preparing antibodies for BMP-7 monomer;
3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference:1. Zeisberg M, Hanai J, Sugimoto H, et al. 2003. Nat Med, 9: 964-8.
2. Phillips FM, Turner AS, Seim HB, 3rd, et al. 2006. Spine J, 6: 500-6.
3. Veerasamy M, Nguyen TQ, Motazed R, et al. 2009. Am J Physiol Renal Physiol, 297: F1238-48.
4. Pauly S, Klatte F, Strobel C, et al. 2012. J Shoulder Elbow Surg, 21: 464-73.

Background:Bone Morphogenetic Protein 7 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-7 is mainly expressed in kidney and bladder. It is also present in developing eyes, brain and ear during embryogenesis. BMP-7 also named osteogenic protein-1 (OP-1) is a potent osteoinductive cytokine and plays role in osteoblast differentiation, SMAD1 production and renal development and repair. Human BMP-7 is synthesized with a signal sequence (29 a.a.), a propeptide (263 a.a.), and a growth factor domain (139 a.a.). The growth factor domain of human BMP-7 shares 98 % a.a. sequence identity with mouse and rat BMP-7.

SDS-PAGE: