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重组人GH

产品名称: 重组人GH
产品型号: PP1002119-250μg
产品展商: PERFEMIKER
产品价格: 2650.00 元
会员价格: 0.00 元
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简单介绍

名称:重组人GH 货号:PP1002119 规格:250μg


重组人GH  的详细介绍

名称:重组人GH1

Synonyms:GH1, GH, GHN, GH-N, hGH-N,Pituitary Growth Hormone, Growth Hormone 1, Somatotropin.

Species:9

Accession:P01241

GeneID:2688

Source:Escherichia coli.

Molecular Weight:Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids

Quantity:20µg/100µg/1000µg

AA Sequence:FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.

Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:Lyophilized from a 0.2 µm filtered concentrated solution with 20mM PB, pH 7.0, with 3% Mannitol.

Endotoxin:Less than 1 EU/µg of rHuGH as determined by LAL method.

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference:1. Goffin, V., K.T. Shiverick, P.A. Kelly, et al. 1996. Endocr Rev, 17: 385-410.
2. Tannenbaum, G.S., W. Gurd, and M. Lapointe. 1998. Endocrinology, 139: 3871-5.
3. Welniak, L.A., R. Sun, and W.J. Murphy. 2002. J Leukoc Biol, 71: 381-7.

Background:Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is a peptide hormone that stimulates growth, cell reproduction and regeneration in humans and other animals. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin and leptin. The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.

SDS-PAGE: