产品资料
  首页 >>> 产品目录 >>> 细胞因子 >>> 蛋白酶

rSAK

如果您对该产品感兴趣的话,可以
产品名称: rSAK
产品型号: PP1002145-500μg
产品展商: PERFEMIKER
产品价格: 3410.00 元
会员价格: 0.00 元
产品文档: 无相关文档
我要买:
产品已经成功添加到购物车
你添加了1件产品 查看购物车

简单介绍

名称:rSAK 货号:PP1002145 规格:500μg


rSAK  的详细介绍

名称:rSAK

Synonyms:Sak42D

Source:Escherichia coli.

Molecular Weight:Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.

Quantity:20µg/100µg/1000µg

AA Sequence:SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK

Purity:> 97% by SDS-PAGE and HPLC analyses.

Biological Activity:Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 5.0 × 104 IU/mg.

Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Endotoxin:Less than 1 EU/µg of rSAK as determined by LAL method.

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference:1. Matsuo O, Okada K, Fukao H, et al. 1990. Blood, 76: 925-9.
2. Collen D, Schlott B, Engelborghs Y, et al. 1993. J Biol Chem, 268: 8284-9.
3. Shishido Y, Matsumoto T, Sakai M, et al. 1994. Biol Pharm Bull, 17: 1060-4.
4. Ohlenschlager O, Ramachandran R, Flemming J, et al. 1997. J Biomol NMR, 9: 273-86.

Background:Staphylokinase is an amino acid enzyme secreted by several species of streptococci. It is a 16kDa potent plasminogen activator that converts plasminogen into plasmin which can digest fibrin the major constituent of blood thrombi. SAK forms 1:1 complex with plasmin, which is a positive feedback of producing other complexes. Recent studies on the thrombolytic potential of recombinant SAK in achieving early perfusion in myocardial infarction and in the dissolution of platelet-rich clot have clearly established its immense utility in clinical medicine as a thrombolytic agent and suggested that it can be developed as a potent clot-dissolving agent.

SDS-PAGE: