名称：rSAK
Synonyms：Sak42D
Source：Escherichia coli.
Molecular Weight：Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.
Quantity：20µg/100µg/1000µg
AA Sequence：SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK
Purity：> 97% by SDS-PAGE and HPLC analyses.
Biological Activity：Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 5.0 × 104 IU/mg.
Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation：Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin：Less than 1 EU/µg of rSAK as determined by LAL method.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference：1. Matsuo O, Okada K, Fukao H, et al. 1990. Blood, 76: 925-9.
2. Collen D, Schlott B, Engelborghs Y, et al. 1993. J Biol Chem, 268: 8284-9.
3. Shishido Y, Matsumoto T, Sakai M, et al. 1994. Biol Pharm Bull, 17: 1060-4.
4. Ohlenschlager O, Ramachandran R, Flemming J, et al. 1997. J Biomol NMR, 9: 273-86.
Background：Staphylokinase is an amino acid enzyme secreted by several species of streptococci. It is a 16kDa potent plasminogen activator that converts plasminogen into plasmin which can digest fibrin the major constituent of blood thrombi. SAK forms 1:1 complex with plasmin, which is a positive feedback of producing other complexes. Recent studies on the thrombolytic potential of recombinant SAK in achieving early perfusion in myocardial infarction and in the dissolution of platelet-rich clot have clearly established its immense utility in clinical medicine as a thrombolytic agent and suggested that it can be developed as a potent clot-dissolving agent.
SDS-PAGE：
