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rTEV

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产品名称: rTEV
产品型号: PP1002149-10000IU
产品展商: PERFEMIKER
产品价格: 9360.00 元
会员价格: 0.00 元
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简单介绍

名称:rTEV 货号:PP1002149 规格:10000IU


rTEV  的详细介绍

名称:rTEV

Synonyms:P1 Protease

Source:Escherichia coli.

Quantity:300IU/1000IU/10000IU

Unit Definition:One unit is defined as the amount of enzyme needed to cleave 3 μg of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.

Purity:> 90 % by SDS-PAGE analysis.

Physical Appearance:Clear colorless liquid.

Formulation:A 0.2 µm filtered solution in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM EDTA, 10 mM GSH, with 50 % Glycerol.

Recommended Conditions for Cleavage of a Fusion Protein:A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4 °C incubations are recommended. Reactions at 4 °C will require longerincubation times and/or more rTEV.

Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 6 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after opening.

Reference:1. Dougherty WG, Cary SM, Parks TD. 1989. Virology, 171: 356-64.
2. Harder B, Schomburg A, Pflanz R, et al. 2008. Biotechniques, 44: 765-72.
3. Wu X, Wu D, Lu Z, et al. 2009. J Biomed Biotechnol, 2009: 591923.
4. Taxis CandKnop M. 2012. Methods Mol Biol, 832: 611-26.

Background:TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It is consists of 241 a.a. amino acids with the molecular weight of 27kDa. TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X’, and cleaves between Q (or S)/X’. In this form X and X’ stand for any of the amino acid residues, except that X’ cannot be P. The optimal cleavage site is ENLYFQ/G. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography.