产品资料

reGFP

如果您对该产品感兴趣的话,可以
产品名称: reGFP
产品型号: PP1002225-10μg
产品展商: PERFEMIKER
产品价格: 600.00 元
会员价格: 0.00 元
产品文档: 无相关文档
我要买:
产品已经成功添加到购物车
你添加了1件产品 查看购物车

简单介绍

名称:reGFP 货号:PP1002225 规格:10μg


reGFP  的详细介绍

名称:reGFP

Synonyms:eGFP

Accession:P42212

GeneID:7011691

Source:Escherichia coli.

Molecular Weight:Approximately 26.9 kDa, a single non-glycosylated polypeptide chain containing 239 amino acids.

Quantity:10µg/50µg/1000µg

AA Sequence:MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Endotoxin:Less than 1 EU/µg of reGFP as determined by LAL method.

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference:1. Prendergast FG, Mann KG. 1978. Biochemistry. 17:3448-53.
2. Shaner NC, Steinbach PA, Tsien RY. 2005. Nat Methods. 2:905-9.
3. Phillips GJ. 2001. FEMS Microbiol Lett. 204:9-18.
4. Wong FH, Banks DS, Abu-Arish A, et al. 2007. J Am Chem Soc. 129:10302-3.

Background:Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in short-wave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

SDS-PAGE