产品资料

重组人ATF1/Cyclic AMP-dependent transcription factor ATF-1Protein(C-6His)

如果您对该产品感兴趣的话,可以
产品名称: 重组人ATF1/Cyclic AMP-dependent transcription factor ATF-1Protein(C-6His)
产品型号: PN100113-50ug
产品展商: PERFEMIKER
产品价格: 3520.00 元
会员价格: 0.00 元
产品文档: 无相关文档
我要买:
产品已经成功添加到购物车
你添加了1件产品 查看购物车

简单介绍

重组人ATF1/Cyclic AMP-dependent transcription factor ATF-1Protein(C-6His) 货号:PN100113 规格:50ug; 标签:C-6His; 来源:Human; 表达宿主:E.coli


重组人ATF1/Cyclic AMP-dependent transcription factor ATF-1Protein(C-6His)  的详细介绍

名称:重组人ATF1/Cyclic AMP-dependent transcription factor ATF-1Protein(C-6His);


产品说明(Description)
Recombinant Human Activating Transcription Factor 1 is produced by our E.coli expression system and the target gene encoding Met1-Val271 is expressed with a 6His tag at the C-terminus. 
Accession #: P18846
Known as: Cyclic AMP-dependent transcription factor ATF-1;cAMP-dependent transcription factor ATF-1;Activating transcription factor 1;

制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

质量控制(Quality Control) 
Purity: Greater than 95% as determined by reducing SDS-PAGE.    
Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

背景(Background)
Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

电泳(SDS-PAGE)

FOR RESEARCH USE ONLY