重组人FGF-4

名称：重组人FGF-4

Synonyms：HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3

Species：9

Accession：P08620

GeneID：2249

Source：Escherichia coli.

Molecular Weight：Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

Quantity：5µg/25µg/1000µg

AA Sequence：GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Purity：> 96 % by SDS-PAGE and HPLC analyses.

Biological Activity：Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation：Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.

Endotoxin：Less than 1 EU/µg of rHuFGF-4 as determined by LAL method.

Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reference：1. Galland F, Stefanova M, Lafage M, et al. 1992. Cytogenet Cell Genet. 60:114-6.
2. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.
3. Boulet AM, Capecchi MR. 2012. Dev Biol. 371:235-45.

Background：FGF-4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.

SDS-PAGE：