名称:重组猪IL-8/CXCL8
Synonyms:(Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF
Accession:P26894
GeneID:396880
Source:Escherichia coli.
Molecular Weight:Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.
Quantity:2µg/10µg/1000µg
AA Sequence:ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ
Purity:> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin:Less than 1 EU/μg of rPoIL-8/CXCL8 as determined by LAL method.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference:1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7.
2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62.
3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6.
4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8.
Background:Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis.
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