名称：重组大鼠Tropic1808, His
Source：Escherichia coli.
Molecular Weight：Approximately 29.1 kDa, a single non-glycosylated polypeptide chain containing 304 amino acids, with 6 × His at the N-terminus.
Quantity：10µg/50µg/1000µg
AA Sequence：MSYYHHHHHH DYDIPTTENL YFQAMDPEFM NLAQIAALNQ ISNLNAIRVG QVLKVSNAAG SNNTQNTTQP SAGVPTNTAS STTGYTVKSG DTLSAIAAAN GVSLANLLSW NNLSLQAIIY PGQKLTIQNA NNATVTTPNA PTSTPTVMPS TNGSYTVKSG DTLYGIAAKL GTNVQTLLSL NGLQLSSTIY VGQVLKTTGA PVAGAGTATS TPTPVTPTVS KPAAANGVST AGLSAAQAAW LRTAVVDAQA ATAGTGVLAS VTVAQAILES GWGQSALASA PYHNFNLYLI KVKNTWKLMT LLLS
Purity：> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity：Data is not available.
Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation：Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin：Less than 1 EU/µg of rRtTPC1808, His as determined by LAL method.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reference：1. Liu M, Gu X, Zhang Q, et al. 2006. J Mol Neurosci, 29: 123-9.
Background：Tropic1808 is a candidate chemotropic factor induced by nerve injury. TPC1808 protein, similar to NGF, could promote the expression of NF-H in a time-dependent manner. TPC1808 is the gene related to promotion of nerve growth, and both the TPC1808 gene and the TPC1808 recombinant protein up-regulate the expression of NF-H in PC12 cells.